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Author : Ronald L. Cihlar, Richard A. Calderone
Date : 2009-02-05
Page : 218
Rating : 4.0
Reviews : 2
Category : Book
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Candida Albicans Methods and Protocols Methods in ~ In Candida Albicans Methods and Protocols expert researchers explore these exciting new insights focusing on the study of medically important fungi and Candida spp in particular Chapters examine critical aspects of molecular methods providing information on reporter gene assays transformation gene expression in vivo and methods for largescale gene disruption
Candida Albicans Methods and Protocols Ronald L ~ Methods and Protocols Provides current methods for experimentation with Candida albicans Applies Immunological Molecular Genetic and Mircobiological approaches Topics include evaluation of vaccines virulence factor determinants biofilms and host cell responses strain typing and others
Candida Species Methods and Protocols Richard ~ Written for the Methods in Molecular Biology series chapters include introductions to their respective topics lists of the necessary materials and reagents stepbystep readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls
Candida albicans methods and protocols in SearchWorks ~ Candida Albicans Methods and Protocols Table of Contents Preface Contributors Immunogical methods 1 Isolation of dendritic cells DC from human blood for in vitro interaction studies with fungal antigens T Sreevalsan 2 Detection and quantitiaton of antifungal SlgA antibodies in body fluids Michael F Cole
Candida albicans SpringerLink ~ Authoritative and cutting edge Candida Albicans Methods and Protocols is an invaluable source of methods for investigators in the exhilarating fields of medical and molecular mycology Keywords DNA Disease models of candidiaisis FISH Gene expression in vivo Proteomics Reporter gene assays Toxin Transformation biofilm gene expression genes molecular biology
Method for CRISPRCas9 Mutagenesis in Candida albicans ~ Candida albicans is the most prevalent and important human fungal pathogen The advent of CRISPR as a means of gene editing has greatly facilitated genetic analysis in C albicans Here we describe a detailed stepbystep procedure to construct and analyze C albicans deletion mutants This protocol uses plasmids that allow simple ligation of synthetic duplex 23mer guide oligodeoxynucleotides
Candida Species Methods and Protocols Methods in ~ Written for the Methods in Molecular Biology series chapters include introductions to their respective topics lists of the necessary materials and reagents stepbystep readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls
Candida albicans Current Biology ~ C albicans thought to be an obligate diploid can form true filamentous hyphae in addition to the budding yeast and pseudohyphal elongated yeast cells seen in other Candida species and in the model yeast Saccharomyces cerevisiae
Cell biology of Candida albicans–host interactions ~ The cell biology of Candida albicans is adapted both for life as a commensal and as a pathogen C albicans can either downregulate or upregulate virulence properties in the human host This fungus modulates the activity of phagocytes to enable its own survival
Candida albicans Biology molecular characterization ~ Although C albicans is the most frequently isolated Candida species in IC nonalbicans Candida NAC species are also commonly detected Multilocus enzyme electrophoresis MLEE fragment length polymorphism RFLP electrophoretic karyotyping EK and random amplified polymorphic DNA RAPD multilocus sequence typing MLST are known as an efficient technique used for molecular typing of Candida species
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